could you please read this article and answer these questions

Could you please read this article and answer these questions? Each answer should be 4 -5 sentences.

1- What are the independent and dependent variables? What are the controls in this experiment? Any possible confounding factors?

2- As a follow up to this experiment, what changes would you make?

3- What were some limitations on the e-CSF extractions from the rats embryo?

4- Why are Adipose tissue-derived stem cells (ADSCs) important in the growth of the organism?